Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: Monomers used in the synthesis of PBPLP polymer.

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Polymer

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: Schematics of RGD functionalized, DOX loaded, fluorescence emitting crosslinked PBPLP nanogels. (Step 1) Photocrosslinkable fluorescent polymer (PBPLP) crosslinked into fluorescent nanogels in the presence of a photo-initiator, acrylic acid, and UV light; (Step 2) PBPLP nanogels can be surface functionalized with biologically active molecules (herein, RGD peptide) using EDC/sulfo-NHS chemistry; (Step 3) Doxorubicin (anti-cancer drug) can be encapsulated within these soft particles to create surface functionalized, fluorescence emitting drug delivery vehicles.

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Fluorescence, Polymer

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: A) Particle size distribution of PBPLP nanogels recorded by Nanosight LM20, spectrum in the inset represents the 3D plot of particle population resolved in terms of relative intensity and particle concentration. B) micrograph of nanogel morphology observed under TEM (scale bar = 200 nm), C) Nanogel size stability evaluation in PBS (pH 7.4) for a two week time period, and D) Zeta potential evaluated by a zeta potential analyzer.

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Concentration Assay, Zeta Potential Analyzer

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Polymer

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: A) Cytocompatibility of PBPLP nanogels against PC3 cells, B-C) Fluorescent micrograph of PC3 cells labeled with PBPLP nanogel after 8 h of incubation at 1000 μg/ml at 5× (B) and 20× magnification (C).

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Labeling, Incubation

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: A) Particle size distribution of PBPLP nanogels. B) Drug release profiles of PBPLP/DOX nanogels in 0.1 M acetic acid (pH 5.2) and 0.1 M phosphate buffer (pH 7.4). C) Pharmacological activity of PBPLP/DOX against prostate cancer cell lines (PC3). Figure legend: Media = pure media (negative control); PBPLP = 50 μg/ml nanogel (DOX-free control); PBPLP(DOX1, 2, or 3) = 5, 25, or 50 μg/ml respectively of nanogel loaded with 20 wt% of DOX; DOX = 10 μg/ml DOX (positive control). D) Light microscopy of PC3 cells incubated with PBPLP/DOX3 at 12 h (top left), 24 h (right), and 48 h (bottom left).

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Activity Assay, Negative Control, Control, Positive Control, Light Microscopy, Incubation

Journal: Bioactive Materials

Article Title: Highly photostable nanogels for fluorescence-based theranostics

doi: 10.1016/j.bioactmat.2017.03.001

Figure Lengend Snippet: Cellular internalization of PBPLP/DOX and delivery of DOX to the nucleus. Bright field and fluorescent micrograph of cellular uptake for (A) PBPLP nanogels, (B) PBPLP/DOX (50 μg/ml), and (C) free DOX (1 μg/ml) at 24 h. PBPLP nanogels stain the cytoplasm and free or released DOX stains cell nuclei. Scale bar 50 μm.

Article Snippet: A) Excitation and emission spectra of PBPLP polymer in water, B) Emission spectra of PBPLP polymer and nanogels in solution, C) Intensity-absorbance curve of PBPLP nanogels for quantum yield measurement, and D) Fluorescent micrograph of PBPLP nanogels obtained by cytoviva (scale bar = 500 nm).

Techniques: Staining